Add Actual Suggestions From A Nutritionist

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Make certain all the purification buffers are at 4 °C and carry out the protein purification in the chilly room to keep away from protein denaturation. WORD: After the coupling of the first C-terminal amino acid of all peptides to the amino group of the membrane, the amino teams between the spots and likewise among the amino groups within the spot areas do not form bonds with the amino acids. OBSERVE: This avoids the coupling of amino acids in the further cycles with the membrane instead of the growing peptide chain.
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